You can run wxGlade without parameters to start the GUI with an empty project as follows:
wxglade
Run wxGlade GUI on an existing project specifying the
      .wxg file as follow:
wxglade <WXG File>
If you only want to generate the code without starting the GUI,
      use the -g or --generate-code option
      with the language as argument as follows:
wxglade -g <LANGUAGE> <WXG File>
wxglade --generate-code=<LANGUAGE> <WXG File>
Possible values for LANGUAGE are "XRC", "python", "perl", "lisp" or "C++".
You can also specify the destination of the generated code with
      -o or --output option:
wxglade -g <LANGUAGE> -o <DESTINATION> <WXG File>
The DESTINATION argument can be a file or a directory.
Complete command line description:
# wxglade --help
Usage: wxglade <WXG File>             start the wxGlade GUI
 or:   wxglade <Options> <WXG File>   generate code from command line
 or:   wxglade --version              show programs version number and exit
 or:   wxglade -h|--help              show this help message and exit
Options:
  --version             show program's version number and exit
  -h, --help            show this help message and exit
  -g LANG, --generate-code=LANG
                        (required) output language, valid languages are: C++,
                        XRC, lisp, perl, python
  -o PATH, --output=PATH
                        (optional) output file in single-file mode or output
                        directory in multi-file mode
Example: Generate Python code out of myapp.wxg
   wxglade -o output.py -g python myapp.wxg
Report bugs to:    <wxglade-general@lists.sourceforge.net> or at
                   <https://sourceforge.net/projects/wxglade/>
wxGlade home page: <http://wxglade.sourceforge.net/>
Use wxglade.pyw instead of wxglade on Microsoft Windows.
Parameters shown in angle brackets (e.g.,
        “<parameter>”) are
        required.
Parameters shown in angle brackets (e.g.,
        “<parameter>”) are
        required.